This 3-day hands-on workshop will begin with a basic introduction of concepts related to gene regulation followed by a description of three common approaches used to interrogate chromatin biology: ChIP-seq, CUT&RUN and ATAC-seq. This workshop will provide participants with important considerations for setting up a successful ChIP-seq/CUT&RUN/ATAC-seq experiment and describe the detailed workflow for analyzing ChIP-seq data. For each step of the workflow there will be additional information describing how parameters might change for CUT&RUN-seq and ATAC-seq. The workshop will instruct participants on how to efficiently manage and analyze high throughput sequencing data starting from sequence reads through to peak calling, using tools and software available on the HMS-RC’s high performance compute cluster.

Prerequisites:

This is one of our advanced workshops, and requires registants to have attended the following Basic workshop offered by our training team in the last 6-8 months:

• Introduction to command-line interface workshop