Speaker:Jeremy Muhlich

Topic: Image processing, visual analytics, and data sharing for whole-slide imaging and spatial profiling of tissues and tumors

When: Monday, March 11, 2024, 10 AM – 11 AM ET

Where: Gordon Hall, 106 Waterhouse Conference RoomTime (Harvard ID required)

Abstract: Spatial ‘omics has emerged as a breakthrough technology for understanding the types and states of cells in the intact environment of tissues and tumors. High-plex immunofluorescence microscopy is particularly effective because it builds on two centuries of histopathology and tissue biology, exploits the latest advances in computational microscopy, and links changes in protein levels to changes in morphology at the subcellular level. This talk will describe the overall approach and specific tools used in the Laboratory of Systems Pharmacology covering the entire lifecycle of high-plex whole-slide imaging: 1) Extract quantitative single-cell data from extremely large whole-slide tissue images via an automated pipeline (MCMICRO); 2) Support visual analytics tools directed by human experts to interpret nuanced tissue features and subcellular morphologies beyond the current capability of computational methods (Minerva Analysis); and 3) Enable effective public release of integrated datasets by presenting images and analysis results together in a novel web-based storytelling interface (Minerva Story).

Virtual: Zoom link