Whole-genome sequencing analysis of fluoroquinolone resistance acquisition in Mycobacterium tuberculosis
Project Status
Ongoing
Project Deliverables
Wrangle data to construct fully-processed & analysis-ready large WGS MTB sample constructed phylogenies that relate thousands of MTB isolates from different genetic backgrounds dated phylogenies and identification of key mutations based of phylogeny dating tools, identification of FLQ antibiotic resistance emergence in time and geographically
Collaborator Name
Maha Farhat, MD MSc
HMS Department
Biomedical Informatics
Project Description
CCB works with the Farhat lab on a large-scale whole-genome sequencing (WGS) analysis of fluoroquinolone resistance (FQ-R) acquisition in Mycobacterium tuberculosis. This will involve (1) processing of WGS data of 32k genetically diverse M. tuberculosis genomes, (2) reconstructing phylogenetic models that relate diverse strains based on their genome sequences, and (3) identifying key mutations that have contributed to FQ-R emergence and spread.