During this online hands-on workshop, participants will learn:
• How to design a bulk RNA-seq experiment
• The steps involved in taking raw RNA-sequencing data (Illumina) and generating a count (gene expression) matrix
• How to compute and assess QC metrics at every step in the workflow
• How to automate a simple workflow on the cluster using shell scripting
• How to effectively use HMS-RC’s high performance compute cluster (O2) for data management and analysis

Pre-requisite:

The Introduction to command-line interface (shell) workshop