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Bulk RNA-seq with R/Bioconductor

April 14, 2023 @ 1:00 pm - 4:00 pm

This single-session workshop will provide biologists with practical statistical analysis skills to perform rigorous analysis of high-throughput genomic data, specifically in the context of bulk RNA-seq analyses. This workshop focuses on differential analysis; data collection, experimental design, read mapping, and count computation will NOT be covered deeply.  Participants will learn how to:

  • Work with genomic region data in Bioconductor. 
  • Normalize and perform quality-control analysis on bulk RNA-seq data. 
  • Conduct and interpret a differential expression analysis with DESeq2 and other Bioconductor packages.
  • Define and acquire data for gene sets and pathways.
  • Perform permutation-based gene set enrichment analysis.

This workshop assumes basic familiarity with genomics and with R programming.


April 14, 2023
1:00 pm - 4:00 pm
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